FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_659t0_sigw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_659t0_sigw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734434
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT208651.2029861038240717TruSeq Adapter, Index 13 (97% over 38bp)
CTTTAAGAAGAAAGTTTTGTAAGAGTTTTCGTCTTGAAAGTTTGTTAAGA24980.14402392942020278No Hit
CCGCGGTTTCCTTCAACGATTTCAAAAGAAACAGCTTGGCCTTCTTCTAA22850.1317432661029477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTCC32050.059.84783621
AATCTCG28550.058.60778441
CTCCAGT33200.057.98398224
GCACACG33400.057.73657211
GTATGCC28400.057.5515447
AGTCACA33400.057.52728
ACACGTC33550.057.37577413
CACACGT33700.057.12038812
TCCAGTC33800.057.0565925
CACGTCT33950.056.90595214
ACTCCAG34050.056.53650723
CCAGTCA34200.056.2869226
AGCACAC34550.055.916110
CTCGTAT29450.055.73729744
CGTATGC29650.055.36133246
GTCACAT34600.055.32954429
TGAACTC35100.055.0462720
CAGTCAC35100.054.94021227
AACTCCA35300.054.43692422
TCGTATG30150.053.978945
Produced by FastQC (version 0.11.2)