FastQCFastQC Report
Tue 5 Jun 2018
H3WGNAFXY_n01_659t-1_sigw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WGNAFXY_n01_659t-1_sigw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4529469
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT326340.72048180482083TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG231460.5110091271184326TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT62310.13756579413613385TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG64400.064.61816443
ATCGGGG79850.061.4952342
ACTCCGG89750.060.13506332
CGGGGGT40050.059.16226644
GTCACTC91800.058.82705729
CACTCCG91900.058.80437531
TCACTCC92100.058.6386730
GAACTCC92900.058.24545321
AGTCACT93750.057.67811628
TCCGGAG93950.057.56041334
CTCCGGA94050.057.46009433
CTCCAGT94850.057.121824
CCAGTCA95350.056.96908626
ACTCCAG95750.056.7676523
CACACGT96200.056.50085412
TGAACTC96550.056.1885320
CAGTCAC96450.056.17234827
ACACGTC96800.056.15064613
CACGTCT97200.055.70352614
GCACACG97850.055.47657411
Produced by FastQC (version 0.11.2)