FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l04_n01_9R_1-2_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l04_n01_9R_1-2_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39215898
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACACAGATCTCGTAT1940020.49470242910158524TruSeq Adapter, Index 23 (97% over 37bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG618320.15767074873562756No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACACAGATCGCGTAT562220.14336532597060508TruSeq Adapter, Index 23 (97% over 37bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC537390.13703371015499888No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA534870.13639111362437753No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACACAGATCTGGTAT506640.12919250248967906TruSeq Adapter, Index 23 (97% over 37bp)
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG442800.11291339038060533No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG419080.10686482303682043No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG398270.10155830168672919No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)