FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l04_n01_9G_1-12_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l04_n01_9G_1-12_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24278101
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAATCTCGTAT572270.2357144819522746TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAATCTCGTTT568320.2340875013247535TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAATCTCGGTT401900.16554013017739733TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAATCTCGGAT276430.11385981135839249TruSeq Adapter, Index 12 (97% over 37bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT257300.10598028239523347No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT254970.1050205697719109No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)