FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l04_n01_8G_1-12_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l04_n01_8G_1-12_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23721384
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAAGATCTCGTAT2232420.9411002326002563TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAAGATCGCGTAT856720.3611593657435839TruSeq Adapter, Index 1 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAAGATCTCGTA363780.15335530169740516TruSeq Adapter, Index 1 (97% over 37bp)
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT302100.12735344615643No Hit

[WARN]Adapter Content

Adapter graph