FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l04_n01_7R_1-8_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l04_n01_7R_1-8_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12156411
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGTGCATCTCGTAT1074720.8840767229735816TruSeq Adapter, Index 3 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGTGCATCGCGTAT436240.35885591561522556TruSeq Adapter, Index 3 (97% over 38bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC201040.16537775828737608No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG167940.13814932713281905No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA156940.12910060378840432No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG134860.11093734820252457No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGTGCATCTCGTA134800.11088799152973686TruSeq Adapter, Index 3 (97% over 38bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT129950.10689832714606311No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)