FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l04_n01_7R_1-2_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l04_n01_7R_1-2_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36500268
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAGGACATCTCGTAT1937900.5309276085315319TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAGGACATCGCGTAT769310.2107683154545605TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAGGACATCTCGTA435720.11937446596282526TruSeq Adapter, Index 22 (97% over 37bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC424640.11633887181321519No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG407480.11163753646959525No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA371690.10183212901340888No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)