FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l04_n01_5R_1-8_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l04_n01_5R_1-8_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15199858
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGTAACATCTCGTAT2052711.3504797215868727TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGTAACATCGCGTAT702850.462405635631596TruSeq Adapter, Index 11 (97% over 36bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC259930.17100817652375436No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG227460.14964613485204928No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA226720.14915928819861343No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG177790.11696819799237598No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT158730.10442860716198797No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGTAACATCTCGTA155280.10215884911556411TruSeq Adapter, Index 11 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)