FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l04_n01_2R_1-12_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l04_n01_2R_1-12_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19152101
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGAGATATCTCGTAT557400.2910385654294534TruSeq Adapter, Index 1 (97% over 36bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC243490.12713487674276572No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA219440.11457750771051176No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGAGATATCGCGTAT208700.10896976785993348TruSeq Adapter, Index 1 (97% over 36bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG204320.10668281250187643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)