FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l04_n01_1R_1-8_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l04_n01_1R_1-8_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11481192
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCTCGTAT1445421.2589459352304186TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCGCGTAT478580.4168382516379832TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCTGGTAT214840.18712342760229078TruSeq Adapter, Index 4 (97% over 38bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG187130.1629883029566965No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCTCGTTT183660.15996596869035898TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCTCGGAT154470.13454177928563515TruSeq Adapter, Index 4 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGACCTTGATCTCGTA119930.10445779497459846TruSeq Adapter, Index 4 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)