FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l04_n01_1R_1-12_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l04_n01_1R_1-12_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15827339
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCATTGCATCTCGTAT1757921.1106857570941016TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCATTGCATCGCGTAT758590.4792909281844535TruSeq Adapter, Index 6 (97% over 37bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT318730.2013793980150422No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCATTGCATCTCGTA269560.17031289972369962TruSeq Adapter, Index 6 (97% over 37bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC178200.11258999380755035No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG160290.10127413079355917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)