FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l04_n01_12R_1-2_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l04_n01_12R_1-2_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55341628
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGTTT1316000.23779567886943986TruSeq Adapter, Index 11 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGGTT1162140.2099938223718319TruSeq Adapter, Index 11 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGTAT1159860.20958183593731647TruSeq Adapter, Index 11 (97% over 39bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC783790.14162756469686796No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA693990.1254010814427071No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGGAT656040.11854367565768034TruSeq Adapter, Index 11 (97% over 39bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG642390.11607717792472604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)