FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l04_n01_10G_1-2_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l04_n01_10G_1-2_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24955826
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCTCGTAT18010467.216936037300468TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCTCGTA6247792.503539654427788TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCGCGTAT5687052.278846630842834TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCTCGTTT3164711.2681247256652615TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCGCGTA2212630.8866186196361523TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCTCGGAT1993990.7990078148485248TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCTCGTT1523780.6105908896784261TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCTGGTAT1097990.4399734154261214TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCGCGTTT1084330.43449974366706995TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCGCGGAT1051800.42146471128625435TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCTCGGA811460.32515854213761547TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCGCGTT579250.2321101293140928TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCTCGGTT576330.23094006185168944TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCTGGTA536710.21506400950223004TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCGGGTAT410040.16430632269995793TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCTGGTTT355870.14259996844023515TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCGCGGA341010.1366454470391002TruSeq Adapter, Index 2 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCTCGTATG334930.13420914218587676TruSeq Adapter, Index 2 (97% over 35bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCTCGTA319620.12807430216896049TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCGCGGTT296760.11891411648726835TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCTCGGT287440.11517951760041922TruSeq Adapter, Index 2 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265900.10654826652501904No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCTCGTGT260490.10438043605529225TruSeq Adapter, Index 2 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)