FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l03_n01_8G_1-16_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l03_n01_8G_1-16_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11051582
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAAGCAATCTCGTAT372900.3374177561185358TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAAGCAATCTCGTTT369660.334486049146629TruSeq Adapter, Index 10 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282530.255646657645937No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAAGCAATCTCGGTT217990.1972477786438177TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAAGCAATCTCGGAT144790.13101291742666346TruSeq Adapter, Index 10 (97% over 36bp)
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT125410.1134769664650726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)