FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l03_n01_5R_1-16_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l03_n01_5R_1-16_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9116760
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338600.3714038759383816No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATAGCATCTCGTAT262570.2880080203932099TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATAGCATCTCGTTT228680.25083472637208837TruSeq Adapter, Index 15 (97% over 36bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG120670.13236061934283672No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATAGCATCTCGGTT110050.12071174408452125TruSeq Adapter, Index 15 (97% over 36bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC98850.10842667789872718No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)