FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l03_n01_1R_1-16_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l03_n01_1R_1-16_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10847851
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG189980.17513146152173367No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCTCGGT164700.15182730662506333TruSeq Adapter, Index 16 (97% over 38bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC156830.14457241346696226No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA127530.11756245545776763No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT114220.10529274415734508No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGTCTGAATCTCGTT111940.10319094537710741TruSeq Adapter, Index 16 (97% over 38bp)
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG109270.10072962838446066No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)