FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l03_n01_15R_1-16_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l03_n01_15R_1-16_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14404911
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAGTACATCTCGTAT867400.6021557509102278TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAGTACATCGCGTAT403830.2803418917340066TruSeq Adapter, Index 7 (97% over 37bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG202430.140528462827712No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC169220.11747382541967806No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGAGTACATCTCGTA148030.10276356445381718TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)