FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l03_n01_12G_1-16_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l03_n01_12G_1-16_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3428472
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCTCGTAT38520411.235442494499006TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCGCGTAT1669724.870157901245803TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCTCGTTT550271.6050007116873055TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCTCGTA468311.3659437790362587TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCGCGTTT254120.7412048282733533TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCGCGTA230840.6733028591162477TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCTCGGAT230500.6723111636904137TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCGCGGAT213600.6230180675239583TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCTCGTT90730.2646368411350596TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCTGGTAT68920.2010224963190599TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCTCGGTT62740.18299697357890043TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCGCGGTT55440.16170468943599364TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCTCGTGT48620.14181244589426428TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCGCGTT44770.13058295357232028TruSeq Adapter, Index 2 (97% over 36bp)
CGCACATTCCGCTTCTACCGGGGGGGTCGAGCTGAGTGGATGCGAATCTG43620.12722868963199935No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCTCGTAA42940.1252452987803313TruSeq Adapter, Index 2 (97% over 36bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCTCGTA42480.12390359320420291TruSeq Adapter, Index 11 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCGCGTGT37370.10899899430416816TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAATGTCATCTCGGA35710.1041571872250962TruSeq Adapter, Index 2 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)