FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l03_n01_11R_1-16_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l03_n01_11R_1-16_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13997953
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGGTTATCTCGTAT1917641.3699431624038172TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGGTTATCGCGTAT933280.6667260563026608TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGGTTATCTCGTA447670.31981104665803634TruSeq Adapter, Index 2 (97% over 36bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG267070.19079218225693428No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGGTTATCGCGTA233410.16674580919081528TruSeq Adapter, Index 2 (97% over 36bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC203490.145371255354265No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA175440.12533261113249916No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG147370.10527967910736663No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG144550.10326509883266503No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)