FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l03_n01_10R_1-16_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l03_n01_10R_1-16_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13914497
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGTGCATCTCGTAT1233070.8861764819813465TruSeq Adapter, Index 3 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGTGCATCGCGTAT555440.3991807968336908TruSeq Adapter, Index 3 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGTGCATCTCGTA258790.18598588220616238TruSeq Adapter, Index 3 (97% over 38bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG196260.14104713954086878No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC159580.11468614352354957No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA139480.10024077765800662No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)