FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l03_n01_10R_1-15_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l03_n01_10R_1-15_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14060499
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGACCGTATCTCGTAT258950.1841684281617601TruSeq Adapter, Index 7 (97% over 37bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG249860.17770350824675568No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC215970.15360052299708565No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA171610.1220511448420145No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG160530.11417091242636553No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG150340.10692365896829124No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG146810.10441307950734892No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG145430.10343160651695221No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)