FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l03_n01_10R_1-14_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l03_n01_10R_1-14_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20466058
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG327490.16001615943822695No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAGGACATCTCGTAT291530.14244560432693TruSeq Adapter, Index 22 (97% over 37bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC280320.13696824273633934No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG234750.11470210824185098No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA229440.1121075685410449No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG209590.10240858303049859No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)