FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l02_n02_3G_2-4_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l02_n02_3G_2-4_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27620842
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2800691.0139770539942266No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT509490.184458533161299No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT483870.17518292889116124No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC470840.17046547675845652Illumina Single End PCR Primer 1 (100% over 50bp)
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA306010.11078952625701997No Hit
CCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTC278490.10082603564366358No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG278030.10065949473951591No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)