FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l02_n02_10G_2-4_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l02_n02_10G_2-4_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.15360983102918588No Hit
AGGAAATATGGCCAAAAGGCCGTCAAGAACAACAAGTTCCCTAGGAGTTA20.10240655401945725No Hit
CGCGCTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTGGGAGGTGATG20.10240655401945725No Hit
CACGACGAGCAAATACTTTTGAAAAGCTGGAGAAAATTGGCCAAGGAACA20.10240655401945725No Hit
CGACAACAACCATGAGTTTATTCCCTTGTGAAGCTTCTAACATGGATTCC20.10240655401945725No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20.10240655401945725No Hit
AAAGAATCCCATTTTCGGGCCAGGCAATGCCTTCTGTCTGGTTGGTACAT20.10240655401945725No Hit
CTTCAATGTCAAAAGTTTTTCCAAAAAATTCATAACTATTTCTTCCGGTC20.10240655401945725No Hit
GAAACGTATAAAGCTGATATAACGATAGATCTGAAGAAGCATCATGTTCC20.10240655401945725No Hit
TAACAAAACAACAATTGGTGCCTACATTGAGTGGGTTGATATGTTGTCAA20.10240655401945725No Hit
CTGCACTTGACCCTGACCTTGATTCACAACTCTGTACGTTTCTTTCTTAG20.10240655401945725No Hit
ATCGACACTGACAACGGTGATGATGAAACTGATGATGCTGGTGCATTGGC20.10240655401945725No Hit
CTCGGGCTCTTTTGTTGCTGCATGAAGGGCACTTGTATTGTTTGATATGC20.10240655401945725No Hit
CTCAAGTACCTCACCGGCTCAGCCAAGACCTACGCTAACTTGACTCAGGT20.10240655401945725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)