FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l02_n01_9R_1-18_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l02_n01_9R_1-18_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13175698
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAAGATCTCGTAT3536102.68380468344068TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAAGATCGCGTAT1370291.0400132122032548TruSeq Adapter, Index 1 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAAGATCTCGTA494440.37526664621487227TruSeq Adapter, Index 1 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAAGATCGCGTA230450.17490534467320062TruSeq Adapter, Index 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)