FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l02_n01_6R_2-4_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l02_n01_6R_2-4_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26811776
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGGGT2041590.7614527288307944TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGGTT881280.3286913929163066TruSeq Adapter, Index 16 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT758730.28298386500021483No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCGCGGGT734490.27394306143688507TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGGGG660470.2463357891696544TruSeq Adapter, Index 16 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGGG372060.13876738340645542TruSeq Adapter, Index 16 (97% over 37bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC371870.1386965190220894No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG366840.13682047768860967No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCGCGGGG354450.1321993738870562TruSeq Adapter, Index 16 (97% over 37bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA297450.11094005857724606No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)