FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l02_n01_5R_2-13_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l02_n01_5R_2-13_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14297604
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGCTAGATCTCGTAT9238566.4616141277937205TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGCTAGATCGCGTAT3280322.294314487937979TruSeq Adapter, Index 12 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGCTAGATCTCGTA981380.6863947273962826TruSeq Adapter, Index 12 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGCTAGATCGCGTA403890.28248789097809673TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGCTAGATCTGGTAT228100.15953722036223691TruSeq Adapter, Index 12 (97% over 37bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG152160.10642342591108273No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGCTAGATCTCGTATG145220.10156946576503308TruSeq Adapter, Index 12 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)