FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l02_n01_4G_2-13_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l02_n01_4G_2-13_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15809230
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGGTTATCTCGTAT4414412.7922991821866088TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGGTTATCGCGTAT1822831.1530163075621014TruSeq Adapter, Index 9 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGGTTATCTCGTA442400.27983652587760444TruSeq Adapter, Index 9 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAACGGTTATCGCGTA221550.14013965259535097TruSeq Adapter, Index 9 (97% over 37bp)
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT162860.1030157699015069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)