FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l02_n01_3R_2-4_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l02_n01_3R_2-4_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33015906
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTCGGTT917010.27774794367296785TruSeq Adapter, Index 9 (97% over 36bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG656440.19882537828887686No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC579670.17557294959587055No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCTCGTTT574590.17403429728688954TruSeq Adapter, Index 9 (97% over 36bp)
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA444390.13459875976143135No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT393870.11929704427920287No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGCCAATCGCGGTT374660.11347863663047746TruSeq Adapter, Index 9 (97% over 36bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG350120.10604585559457311No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG345560.1046647031282437No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG343740.1041134536789631No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)