FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l02_n01_3R_2-13_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l02_n01_3R_2-13_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26516434
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACATCTCGTAT2268380.8554619373027308TruSeq Adapter, Index 15 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAACACATCGCGTAT785450.29621252993520925TruSeq Adapter, Index 15 (97% over 36bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG317370.11968803950033402No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC305660.1152719102425311No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)