FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l02_n01_3G_2-4_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l02_n01_3G_2-4_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27620842
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCTCGTAT4249131.5383781566108665TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCGCGTAT1505620.5451028610930833TruSeq Adapter, Index 13 (97% over 38bp)
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT530350.19201080111895213No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT512980.1857220717601585No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCTCGTTT367630.13309876650393207TruSeq Adapter, Index 13 (97% over 38bp)
GGCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATG327660.11862781011527455No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCTCGTA293160.10613724230419913TruSeq Adapter, Index 13 (97% over 38bp)
CCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTC290890.10531539914677474No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)