FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l02_n01_15R_2-4_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l02_n01_15R_2-4_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38210176
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCATACATCTCGTAT1291580.3380199033890867TruSeq Adapter, Index 10 (97% over 37bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG667190.17461055400529954No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC558970.1462882557777279No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT507790.13289391810181664No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCATACATCTCGTA460090.12041033257737416TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCATACATCGCGTAT416720.10905995303450056TruSeq Adapter, Index 10 (97% over 37bp)
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT399040.10443291336841788No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA397080.10391996100724582No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG396110.10366610193054332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)