FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l02_n01_14G_2-4_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l02_n01_14G_2-4_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26674939
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCTCGTAT4736461.7756216799596054TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCGCGTAT1656510.6209986084691702TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCTCGTTT917730.3440420238636722TruSeq Adapter, Index 18 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCTCGTA824910.3092453182367165TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCTCGGAT480490.18012787208248163TruSeq Adapter, Index 18 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCGCGTA376370.14109498057333889TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCGCGGAT355520.13327865529514427TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTACACCTATCGCGTTT343940.1289375019751685TruSeq Adapter, Index 18 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)