FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l02_n01_12G_2-4_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l02_n01_12G_2-4_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9597
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCATTACATCTCGTA220.22923830363655312TruSeq Adapter, Index 10 (97% over 37bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG180.1875586120662707No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC180.1875586120662707No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCATTACATCTCGT130.13545899760341773TruSeq Adapter, Index 10 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.12503907471084713No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA110.11461915181827656No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG100.10419922892570596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)