FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l02_n01_10R_2-4_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l02_n01_10R_2-4_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36531788
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1871210.512214184534302No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATCTCGTAT1240370.3395316977094031TruSeq Adapter, Index 15 (97% over 37bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG712900.1951451158098257No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC615220.16840675851945708No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATCGCGTAT516030.14125506257728201TruSeq Adapter, Index 15 (97% over 37bp)
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG434860.11903605703613522No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT408490.11181768601087906No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA401410.10987964782889904No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG380600.10418323899175151No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTGACCATCTCGTA376420.1030390300086051TruSeq Adapter, Index 15 (97% over 37bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG376360.10302260595621544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)