FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l02_n01_10G_2-4_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l02_n01_10G_2-4_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACG30.15360983102918588No Hit
CGATGCTTTTATATGTAATTTTGAATTGATTTATAGGTTTTGGGAATCCC30.15360983102918588No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG20.10240655401945725No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTG20.10240655401945725No Hit
AACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGA20.10240655401945725No Hit
TTGGCCAGAATCTCAACAACCTCTCCGCGGTGAAGTGCGGTCTCGGCGAG20.10240655401945725No Hit
CACAGATCTGGTAGTCTGTGTCATTTTCAAAACATCTTCCTTTGGAAGAG20.10240655401945725No Hit
AACAAACAAAAAGGTGCGTGCCGCACTCACGAGGGACTGCCAGTGATATA20.10240655401945725No Hit
TCTGGATCTGCTGTGACCTTTGTGAGAAGTGGTTCCATGGCAAGTGTGTG20.10240655401945725No Hit
ACGGCTGTGACAATGTTCAAGCCCACTTAGAAGTTGTCGCATGTAACATT20.10240655401945725No Hit
AAGATGTCAAGTACTTTAGCTTGGTAGACAACAAGCGTTTGAAGTCTGGT20.10240655401945725No Hit
TATGCTCGAAGTTTTCGGTGGATTTCTCGATGGGATGCGAATGCACTCCT20.10240655401945725No Hit
GGTGACGGAACCATCAGTTTCTCTGAGTTTGTGTGTGTAATGACTGGTAA20.10240655401945725No Hit
AACTGTGGTTCAATCTGACCATAAAGATGAGGGAGACTACTGATAGATTC20.10240655401945725No Hit
CGCTGCTACAGTTTCAAGCATCATAGCTCGACATCCATATCTCCTCTGGA20.10240655401945725No Hit
GAATTAGCTCGTTTAGCAAACTCCTCCGCTCCCAATTCATCATTTCCATG20.10240655401945725No Hit
GGCATCCAATAATCTCCATAATCAATTCATCTCCAAATATTTTCTCCAAG20.10240655401945725No Hit
ATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCG20.10240655401945725No Hit
CAAAGGTTTGCTCAAACTGTATCGACCTTGCTTCTAAAGAGGTGTTAGAG20.10240655401945725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)