FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l01_n02_5G_1-1_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l01_n02_5G_1-1_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33686687
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT651950.19353342761192277No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT496220.1473044826284045No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA480260.14256670595122636No Hit
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG431370.12805355421267756No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG379200.11256672405927007No Hit
AGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTG352060.10451012888266513No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG342870.10178204820200928No Hit
CCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTTGTACAGCTCGTC340310.10102210407333913No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)