FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l01_n02_2G_1-9_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l01_n02_2G_1-9_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34413323
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT709330.20612075154730045No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT564970.16417188191910442No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA557810.16209129237533962No Hit
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG459320.1334715627433015No Hit
AGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTG431480.12538167267369094No Hit
CGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACG403200.11716392514608369No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGT371570.10797271742691049No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)