FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l01_n02_15G_1-10_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l01_n02_15G_1-10_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13101438
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGA323390.24683550004205643No Hit
CTGGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGAT279650.21344985184069107No Hit
GGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGG231130.17641574917196112No Hit
GGTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCC192570.14698386543522932No Hit
GTGGACAGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCG182330.13916792950514287No Hit
ATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCA175220.13374104430368636No Hit
GGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACA168250.128421017601274No Hit
GGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGG155430.11863583218880248No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT152270.11622388321037735No Hit
GCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATG150270.11469733322403235No Hit
GCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATC144890.11059091376076427No Hit
ATGCGGTTCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTCTT140720.10740805703923492No Hit
CCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGC139780.10669057854565277No Hit
GGGGTCTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCA133670.10202696833736875No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)