FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l01_n02_14G_1-10_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l01_n02_14G_1-10_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37330272
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGA781350.209307341773454No Hit
AGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTG601190.1610462415060892No Hit
CCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGC539130.14442166400501985No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT513980.13768450441507632No Hit
GGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGG415780.11137877591676804No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA410720.110023307625511No Hit
GGGGTCTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCA382710.10252001378398742No Hit
CGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCA380740.1019922919393676No Hit
GGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGG374770.10039305365897146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)