FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l01_n01_4R_1-9_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l01_n01_4R_1-9_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44272238
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG1437930.3247927064360288No Hit
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC1417110.32008998506016345No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA1132780.25586689337909685No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG985970.2227061572988472No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT712280.16088637759853025No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT670330.15141091353908967No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC620600.14017814053131897No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG616350.13921817099013606No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTC613650.13860830798750223No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC602520.13609431716553386No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT581180.13127414069286492No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC561750.12688538582576286No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG560420.12658497182816916No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG558830.12622583028217366No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCT518570.11713209528734464No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA512200.11569327035150108No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC483650.10924453378661364No Hit
CAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCG477400.10783281387310938No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)