FastQCFastQC Report
Sun 21 Apr 2024
H3WG2DSXC_l01_n01_12G_1-9_NEB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3WG2DSXC_l01_n01_12G_1-9_NEB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47413687
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTACAGCTCGTCCTTCTT986750.20811501117810136No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATCACTGGTGGACAGCAAAT928980.19593076573015722No Hit
AGCAAATCGGTCGGGATCTGTAGGACGATCACGATAAGGATCCGATGGTG680150.14345013919714786No Hit
CTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCA573120.12087648868142231No Hit
CTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAGCAGGA514710.10855726111323087No Hit
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCG513250.1082493331514168No Hit
CTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGT497160.1048557982845755No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC491040.10356503175971106No Hit
CGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCG480490.10133993587126011No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)