FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411972
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10640.25826997951317077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATACT551.3098725E-744.5454524
TATAACC350.001254831140.02
AATAGTA459.191623E-538.8888852
TCTGGTC1350.033.7037058
TGTACTG1253.6379788E-1233.65
ACAATAG450.00430528531.11110969
GCCTACT450.00430528531.11110969
GGTCGTG703.4854653E-530.07
ACTGTAC703.4854653E-530.08
CATAATT952.817178E-729.47368616
GGTATAC604.9788086E-429.1666661
ATCTGGT3150.028.888897
TCTGAGG853.9508923E-628.823538
TCCTAAT500.007205084427.9999981
GTATACT500.007205084427.9999982
TAGGGCC906.1629653E-627.2222214
TATACTC700.001225218825.03
TAGTAGG851.3070293E-424.70588335
TCAATAC1001.3959318E-524.4999983
TGTAGGG1151.493936E-624.3478262
Produced by FastQC (version 0.11.2)