FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97
Sequences flagged as poor quality0
Sequence length76
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAA22.0618556701030926No Hit
CTATTTCACTCCCCTCCCGGGGTTCTTTTCGCCTTTCCCTCACGGTACTG22.0618556701030926No Hit
ATGCTCCTTCTATGCCTTGTTGCATGGTTTGCGGTTTCCCATCGGCGTAT22.0618556701030926No Hit
GGGGTGGGAGGGGTGGGCGGTTAGGATGGCGAGTGGGTGACGGGGGCTGT11.0309278350515463No Hit
TTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATG11.0309278350515463No Hit
GAGCTCTGCAGATCTTTTTTGTTTTTTTTTTTTTTTTTTTTTTAAATTTA11.0309278350515463No Hit
GCGCTGGATTCGCTCAGCGCGGAGATCCATCGCGGTGCGCTGTTCGTCGG11.0309278350515463No Hit
CTGCCCCGCGCTTCATTTCACTCTCCGAAAATGCTGATACTCAGGACCAC11.0309278350515463No Hit
ATTTAAAAACCTGCATTAGTGCTCTTAAACGAGTGTTATTGTGGGCCGGT11.0309278350515463No Hit
AGAGTGGACAGGCCGAGGAGCACCGAATTCCAAGAAATGGGTCCGACGTT11.0309278350515463No Hit
GTATCGTTGTGGTCATTTTTAATTTTAATTTCTTGATCCAGCAATCTATT11.0309278350515463No Hit
ACCAAAAAAGTATACTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0309278350515463No Hit
GTGGGATGACGTCGGCGACTGGTCCCGCACCCACGATCTCGCTGTCGCGG11.0309278350515463No Hit
ACTACGTGGTGACGGGACCCGACGGCGCCCTCGTGGGCGTGCCTGTCTCT11.0309278350515463No Hit
CCTTACATGACGAATCCGAATCAAACCAACCCAAACAGCAATCCAACCAA11.0309278350515463No Hit
CCGCAGCAGGGTGCGGCCGGGTTGTCGCTGTTCGCCGCCGAGGTCCGCGA11.0309278350515463No Hit
AAACGGCGGTGGTAACTATAACCATCCTAAGGTAGCGAAATTCCTTGTCG11.0309278350515463No Hit
CATCAATCCAACCCTGATAACCCCTTACCCCTTGAAACCATTGTAGACCA11.0309278350515463No Hit
ACGCAGTATCAAATCAAAAAAAAGCAATAATCCGAAAAAACAAGCAAACA11.0309278350515463No Hit
TCCGTGGGGAGGGCCGCAAAGGCCAGGTGGAGCACCTCGGCGTCGGCTAG11.0309278350515463No Hit
GCGTGCGCAGGCGGTTATGTAAGACAGATGTGAAATCCCCGGGCTCAACC11.0309278350515463No Hit
AAGCCGAAAGATTTCACCAAGTACATCACTTTGGCTACTCTATACAACAT11.0309278350515463No Hit
AAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATG11.0309278350515463No Hit
GCACGGCGAGGCGCTCATCCAGATCGCCGGTACAATACCTGTCTCTTATA11.0309278350515463No Hit
ATCTGCATGTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTT11.0309278350515463No Hit
CTAAAAAGCGAGACGAGTGCTCATCAGAACTACACAGGGAGACGAGTGCT11.0309278350515463No Hit
GGCCTGGTGCTGGTGGGCCACGAACAGGCCGGCGGCAAACACCGGCAGCG11.0309278350515463No Hit
CTCCAGGGGTGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0309278350515463No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.0309278350515463No Hit
CAGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGTATCATT11.0309278350515463Illumina Single End PCR Primer 1 (100% over 29bp)
GTGCAGGGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAG11.0309278350515463No Hit
GAGCACACGTCTCCCAACGGAGATCGGAAGAGCACACGTCTCCCAACGGA11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCAGAACTTAATATAAATATTTTTAATTAAATTAACCAGATGAAAAAA11.0309278350515463No Hit
CCCACTGTAGATCGGAAGAGCACACGTCTCCCACTGTAGATCGGAAGAGC11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTCGCGGGGTGCTCTTCCGATCTGCCCAGGGAGACGTGTGCTCTTCCGAT11.0309278350515463No Hit
TAGGAGTAGAGTCATCATTTCAATCACATGAAAGAAACTAGCTTCTCCCT11.0309278350515463No Hit
GTGCTCTTCCGATCTCGGAGGGGGTGCTCCTGTCTCTTATACACATCTGA11.0309278350515463No Hit
GAGCACACGTCTCCCCTGTCAGATCGGAAGAGCACACGTCTCCCCTGTCA11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTAAACCTCAGAATACACCCTTGCAATAACATCAAGTATCCACAACACGC11.0309278350515463No Hit
TACGATATTACCAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.0309278350515463No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC11.0309278350515463No Hit
TTATTGCACAATCAGTGAAAGATGTTTTAATCGTCAAAAGTGTTGAAATT11.0309278350515463No Hit
ACCATGGTTGCAACGAGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGG11.0309278350515463No Hit
CGTCTCCCTGACCAGATCGGAAGAGCACACGTCTCCCTGACCAGATCGGA11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AGATAACATGCAGATCGTATGGTCTTGTACCGACACAGATCTTTCAAATG11.0309278350515463No Hit
TTGCCAGGGAGACGTGTGCTCTTCCGATCTTGCCAGGGAGACGTGTGCTC11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGAATAATTAGC11.0309278350515463No Hit
TAGTCATTGAGCCCTGACACAATCTAAATTAATAATATTGCCCGTTTATG11.0309278350515463No Hit
AGTCAGGGATTCTGGTTGATCCTGCCAGTAGTTATATGCTTGTCTCAAAG11.0309278350515463No Hit
CGTCTCATCACAGATCGGAAGAGCACACGTCTCATCACAGATCGGAAGAG11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GTCATGCGTGCCATGATTTCTCCTGTTCGAGTTTTGTCATAAAATTCATA11.0309278350515463No Hit
GGTTTAGCCTGATTGTTTTCTCTTCCTGTAGGTACTGAGATGTTTCACTT11.0309278350515463No Hit
CAGTTACGTTGCCGTGGAGAATCCACATCCAGGTGCCGGAATATTAACCG11.0309278350515463No Hit
ACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATAACATGCA11.0309278350515463No Hit
CCTGGGAGCCAATGGCGCCGGAAAGACCACGATCCTGAAGACGATCAGCG11.0309278350515463No Hit
TAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAAATT11.0309278350515463No Hit
GGAGACGTGTGCTCTTCCGATCTAACAGGGGGTGTGCTCTTCCGCTGTCT11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTCCAGTGTGACTGATCATCCTCTCAGACCAGTTACGGATCGTCGCCTT11.0309278350515463No Hit
GATATGGAATCCGAGATCGATCAGGTGGAACAAAGCGCCGACATCAAGCC11.0309278350515463No Hit
GAAGCTTTTCTTGGAAGCATGGCATCAACCACTTCGTCATCTAAAAGACA11.0309278350515463No Hit
GCCCGCGGATCTGGTGCGACAGGGCCGATTGGCTCAGGTGCACACGCTCG11.0309278350515463No Hit
GTCATGATGGACCGCCACGGTGCACAGCATCGTCCGGCGCTCCGGCCGGC11.0309278350515463No Hit
GTGTAGGGCGGGCGACAAAGGGAGATAGAGAGAGAACCGTTGGCGGGAGA11.0309278350515463No Hit
ATAGCGATCTTTTAATTTTATATTAAAATTTTTTGTTAAGAGGAAACCAG11.0309278350515463No Hit
CTGTTCTGCTTTCATTGGTTTTCAGATCAAGAGGTAATGATTAATAGAAG11.0309278350515463No Hit
ATGTCGAGAAGCTGAACGAAGGTATTCGCACGATCAGCGGCATCATCCTG11.0309278350515463No Hit
CAAGTGAGGATCCATGCCTTTCATCCGCCCCCTGCCCGCGCTGGTCCTGG11.0309278350515463No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA11.0309278350515463No Hit
ATCAATCTGTCTTACACACACATGTTCGGACCTGGTAAGTTTTCCCGTGT11.0309278350515463No Hit
GTTGAATTGCACGCAGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG11.0309278350515463No Hit
CCAAGTAACTGTTAACGATCTAAGGAACCATAACTGATATAATGAGCCTT11.0309278350515463No Hit
GTGGGGTGGGTGGCGGGTGGTATGAGGAGGGGGGATGGTGACATGAGGAG11.0309278350515463No Hit
TTTCAAGCGAGTTTTCGCAACGAAATTCGGGGGAATCGAACGGAAAATGC11.0309278350515463No Hit
AGTTAGAGGTTCGAAGGCGATCAGATACCGCCCTAGTTCTAACCATAAAC11.0309278350515463No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCG11.0309278350515463No Hit
GGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTT11.0309278350515463No Hit
ATGGTTACCTTACACTGAATACATAGGTGTATGGAGCGAACCAGGGGAAC11.0309278350515463No Hit
GGTCGAGCAAAGAGACTACGCCGTCAACCCGGCCGGCTTCATCATCCACA11.0309278350515463No Hit
TCCTTATAATGGGACAAACCAACAGGAACGGCTCCACTTACATAAACACA11.0309278350515463No Hit
CCCTTATGCTACGGCCCATTTTACAGGTAGGAAATTGTTGCGGCTCTCTA11.0309278350515463No Hit
CCAGGCGTAGAGGTCGCGTGGGTGGACTCCGAGGAGCCGGGCGGTCTCCG11.0309278350515463No Hit
GAATACCACCACCCTCTTGCGGAAGAAGCGGGCAGTCCGGAGCACGGAAG11.0309278350515463No Hit
TCTTATAAGTGATTAAATACTTGTATCTTTTCATATGTTCCTCCTATTTA11.0309278350515463No Hit
AGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTAG11.0309278350515463No Hit
GTAACATAGTTCGTCCTGATAAGCCTCCCATTTTGAGCGGATGCTTGCAA11.0309278350515463No Hit
GGGAGACGTGTGCTCTTCCGATCTCTTCCGGGGAGACGTGTGCTCTTCCG11.0309278350515463Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGA11.0309278350515463No Hit
CTTTTGATGGTAGTATCTAGGACTACCATGGTTGCAACGGGTAACGGGGA11.0309278350515463No Hit
CTTATACACATCTGACGCTGCCGACGAATTAGACGGTGTAGATCTCGGTG11.0309278350515463No Hit
GCCGCCGAGGCGGTGGACGTTTCCTACAAGGAACTGAAAGCTGTCGTCGA11.0309278350515463No Hit
GAACTAGGGCGGTATCTGAACGCTATCGAACCTCTAACTTTCCAACTTGA11.0309278350515463No Hit
GAGTTAATGACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTT11.0309278350515463No Hit
CTTCCGATCAGACGTGTGCTCTTCCGATCAGACGTGTGCTCTTCCGATCA11.0309278350515463TruSeq Adapter, Index 3 (95% over 24bp)
CCTGCGCCTCTACGAGGACGGCAAGCCGGCGAAGACGCCGGACTATCTGG11.0309278350515463No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC50.070.024
GAGGTGT50.070.019
AGGGGGG50.070.058
TGGTCGC50.070.035
ATCTCGG50.070.028
GTCGCCG50.070.037
GTGTAGA50.070.022
TGCCGAC50.070.05
AGAGGTG50.070.018
TCGGTGG50.070.031
TAAAAAA50.070.050
GTATCAT50.070.043
AAGGGGG50.070.057
AGGTGTA50.070.020
CCGTATC50.070.041
CATTAAA50.070.047
AAAAGGG50.070.055
TCTCGGT50.070.029
GCTGCCG50.070.03
TCGCCGT50.070.038