FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293707
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11540.3929085789579411No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC4880.16615198139642567No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC4380.14912821281072633No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC3890.13244491959674098No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC3740.12733778902103118No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA3620.12325208456046331Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC3250.1106544958070458Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCAAT200.00694303652.507412
AGTCACG200.00694771352.49846611
TGGTACT305.868134E-446.673254
GTACTAA556.675724E-638.1872061
AGTCACC552.9885813E-431.81725569
ATCCGTG552.9885813E-431.8172557
AAAAGTA450.00430032131.11549820
TGTTACC450.00430032131.1154982
TAGGATG450.00430032131.1154985
GATTAGG604.97126E-429.1707821
CGTCCCG500.00720279627.99918210
CTGTGCG500.00720279627.9991829
TTTCGGA1154.8717993E-827.3951681
GGGTAGG657.938613E-426.9268741
AAGTCAC657.946515E-426.9222968
GATCTTT2050.025.6090096
TGTAAGG700.001223374225.0035272
TATCTGT700.001224589424.9992770
CGGTGTA3700.024.59387670
AACTCCG750.001830108823.33265342
Produced by FastQC (version 0.11.2)