FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245585
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8610.3505914449172384No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC3630.14781033043549077No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC3170.1290795447604699No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2990.12175010688763564No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA2690.10953437709957856Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC2640.10749842213490238Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC2630.10709123114196713No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTT150.002222995369.998574
ATAGTGC200.006946119552.4989352
TATATCG400.002415983734.9992872
ATGTACT400.002415983734.9992874
GGGAGGG552.9870088E-431.81753511
TACCTCT450.00430235831.11047740
CCGAATA450.00430235831.11047720
TAGGCAG703.4802746E-529.999395
CTAGGCA755.571226E-527.999434
CCGGGTT657.9423736E-426.9225274
CCCTAGG657.9423736E-426.9225272
AGGATTA1101.01164E-625.4540275
GATTATG1101.01164E-625.4540277
GTCGTAA700.001222501325.00458334
ATGAAGA700.001223954124.9994957
AGGTGTA2250.024.88838270
AGTATCT901.9231343E-423.33285955
CAGGATT1202.157789E-623.3328594
ATTATGG1202.157789E-623.3328598
CCCCGTC750.001829164823.33285720
Produced by FastQC (version 0.11.2)