FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343608
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12800.37251751996461085No Hit
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG11420.33235547484342626No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA10200.2968498987217993No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT9810.28549975553537754No Hit
GTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAG9470.2756047589113175No Hit
CACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCC7860.22874903960326884No Hit
TTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTG7520.21885404297920888No Hit
GACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCAC7420.21594374985448536No Hit
TAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGC6520.18975111173197362No Hit
GTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGA6100.17752788060813485No Hit
TCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGG5930.17258038229610487No Hit
GCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGGTCTAGGAAATAC5500.16006612185979371No Hit
GATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGA5360.1559917114851808No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCG5320.15482759423529138No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAA5280.15366347698540198No Hit
GGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGAGATTG5240.15249935973551254No Hit
CTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAA5170.1504621545482061No Hit
CCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGT4970.14464156829875904No Hit
GGATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATG4810.13998509929920142No Hit
CTTCCGATCTGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTT4760.13852995273683966No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC4720.13736583548695025No Hit
GCAATAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCT4650.1353286302996438No Hit
CATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGG4610.13416451304975438No Hit
GCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTA4580.13329142511233733No Hit
GTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGC4560.13270936648739262No Hit
ACAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAAC4550.13241833717492027No Hit
CTACAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAA4460.12979907336266908No Hit
ATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGGTCTAG4420.12863495611277967No Hit
CCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTG4400.12805289748783497No Hit
GTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACC4300.12514260436311145No Hit
GGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAA4270.1242695164256944No Hit
GGGATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAAT4020.11699378361388559No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC4010.11670275430141323No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC3990.11612069567646854No Hit
TAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATT3920.11408349048916207No Hit
AGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGC3730.10855393355218737No Hit
CAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAACC3730.10855393355218737No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC3700.10768084561477032No Hit
TCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTG3590.10447952317757445No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGTAT200.00694739552.5009853
CATAACA200.006951395452.493344
GGAGAAT350.001255323239.9949268
CATAGAC350.001255323239.9949262
GAGTAAT400.002416858435.00065665
AACTATG400.002418585134.9955669
CTGTGCG601.2151249E-534.995569
TAGGGAT851.0624899E-732.9374
AGACATT901.7523416E-731.11169434
AGGGATA1151.4551915E-930.4309235
GTGTTAG500.00719766928.00459946
TAGAGTC500.00720278728.0005237
TCTTAAG755.5816585E-527.9964492
TAGGCCG500.007207907327.9964495
CTTTAGG500.007207907327.9964491
ATCTGGG4600.027.387837
TTGTGTA3150.026.66716670
GTCCTAG1056.760929E-726.6632861
TCTGGGC3400.025.732038
TTCCATT700.001225710624.99682811
Produced by FastQC (version 0.11.2)