FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences218982
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13350.609639148423158No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCAAG200.006944560452.51
AAACGAC305.8689254E-446.66666444
TGGACAT404.5825695E-543.755
ATCCTAG350.00125354440.01
TGATACA459.177513E-538.888894
ACTTACT400.002415172835.030
GAACGGT400.002415172835.019
TTCGGAA851.0583244E-732.9411772
TACACCG652.0962978E-532.3076935
AACGTAT450.004300920831.11111319
AGATCAT450.004300920831.11111322
ACGCAAG450.004300920831.11111311
GAACGTA450.004300920831.11111318
AGGATAG604.9712823E-429.1666665
TACCCTT500.007197825728.0000025
CCTAGGG500.007197825728.0000022
TTTCGGA1004.3971158E-728.0000021
ACTTAAT500.007197825728.0000022
ATCTACG1403.9108272E-1027.57
TCTAGGG657.938662E-426.9230792
Produced by FastQC (version 0.11.2)