FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227133
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10270.45215798673024177No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTA252.3869403E-455.9999964
CTATAGG200.006944892552.52
TATAACA200.006944892552.54
GTCGACC556.6755056E-638.1818164
GTATTAA601.2122609E-535.01
TCTAAAC400.002415361635.03
TATATAG552.985951E-431.818185
AGTGGAC450.004301255631.11111324
ATATCAT450.004301255631.1111133
GATCTAG2700.029.8148176
TTTCGGA1202.2810127E-929.1666661
CAGCACT604.9718586E-429.1666664
TAGCACT500.007198384527.9999984
CACTCTG500.007198384527.9999987
GTACTAT500.007198384527.9999981
CGTGTAG2300.027.39130670
TGCTTTA657.9395797E-426.92307735
CGATCTG11450.026.288215
ACTTATA808.640645E-526.251
TATAGTT808.640645E-526.255
Produced by FastQC (version 0.11.2)