FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n02_adr3wtzt14s90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n02_adr3wtzt14s90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239816
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14620.6096340527737932No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATAGG200.006945363252.52
TACTTTA350.001253782540.00000440
TACTACT459.1801325E-538.88888532
ATCTGTT459.1801325E-538.8888857
ATCTAGC852.561137E-937.0588267
AACGTAT601.2125434E-535.019
ATTATTC400.0024156335.03
TCATTGT450.00430173131.11110970
ACTAGAT450.00430173131.11110919
TCTCTAA500.00719917328.00000213
TCTACAG500.00719917328.0000023
TCTAAAG500.00719917328.0000022
TTTCGGA1750.028.01
GATCTTC1554.3655746E-1127.0967756
TTATTCC657.9408806E-426.9230774
AATACTA657.9408806E-426.92307730
CCGATCT33050.026.8986364
TAGGGAG1056.7391557E-726.6666664
GATCTCA2900.026.5517236
TTCCGAT34050.026.4170322
Produced by FastQC (version 0.11.2)